MersV1, Main, Exploration, bibRecord, 004919

Molecular analysis of a baculovirus regulatory gene

Identifieur interne : 004919 ( Main/Exploration ); précédent : 004918; suivant : 004920

Molecular analysis of a baculovirus regulatory gene

Auteurs : David D. Carson [États-Unis] ; Max D. Summers [États-Unis] ; Linda A. Guarino [États-Unis]

Source :

Virology [ 0042-6822 ] ; 1991.

RBID : ISTEX:863BD0CEA511A446FC81F3E308E439E7ECD32A00

English descriptors

Teeft :
- Acmnpv, Amino, Amino acid, Amino acid sequence, Amino acids, Amino terminus, Assay, Autographa, Autographa californica, Baculovirus, Binding proteins, Binding regions, Cagt, Cagt motif, Cagt sequence, Carson, Cell culture, Cell extracts, Cells transfected, Cellular transcription factor, Chloramphenicol acetyltransferase, Coding region, Complete gene, Early gene, Error bars, Frugiperda cells, Functional mapping, Gene, Gene expression, Gene product, Guarino, International union, Minimal promoter, Molecular weight, Nucleotide, Nucleotide sequence, Open reading frame, Plasmid, Polyglutamine tract, Polyhedrosis, Polyhedrosis virus, Promoter, Promoter dissection, Promoter sequence, Regulatory sequences, Restriction fragment, Room temperature, Seal site, Spodoptera frugiperda cells, Standard deviation, Temporal expression, Transcription, Transcription initiation, Transcriptional, Transcriptional activation, Transcriptional regulators, Transfected, Transfected plasmids, Transient, Transient assays, Transient expression, Transient expression assays, Vertical axis, Viral, Viral elements, Viral factors.

Abstract

Abstract: To better understand the structure and function of a baculovirus regulatory gene, the nucleotide sequence of IE-N expressed by Autographa californica nuclear polyhedrosis virus was determined. The 2.0-kb Pstl-EcoRV restriction fragment (97.5 to 98.9 mu) encodes the upstream regulatory sequences, open reading frame, and downstream sequences of the immediate early IE-N gene. Using a convient restriction site, the 285-bp promoter of IE-N was divided into two functional regions as defined by transient expression assays of mutant sequences. The sequences of IE-N from −1 to −45 nt encoded a minimal promoter capable of directing low levels of transcription. The minimal promoter was fully responsive to positive regulation by IE-N. The upstream region from −46 to −285 nt contains two direct repeats which increased levels of IE-N gene expression. Computer-assisted translation of the IE-N sequence indicates that this fragment of DNA encodes a single long open reading frame with a predicted molecular weight of 47,000. The amino acid sequence of the predicted protein exhibits three motifs common to transcriptional regulators: a serinethreonine rich region, a proline-rich region, and a polyglutamine tract. IE-N autoregulates its own expression and stimulates both IE-1 and IE-0 in transient assays. The stimulation of IE-1 may account for the augmenting activity of IE-N in the IE-1-mediated trans-activation of the 39K promoter.

Url:

https://api.istex.fr/ark:/67375/6H6-JJHP57FV-V/fulltext.pdf

DOI: 10.1016/0042-6822(91)90671-W

Affiliations:

États-Unis

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Le document en format XML

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<term>Restriction fragment</term>
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<term>Seal site</term>
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<term>Transcriptional activation</term>
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<term>Transfected plasmids</term>
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<term>Transient assays</term>
<term>Transient expression</term>
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<front><div type="abstract" xml:lang="en">Abstract: To better understand the structure and function of a baculovirus regulatory gene, the nucleotide sequence of IE-N expressed by Autographa californica nuclear polyhedrosis virus was determined. The 2.0-kb Pstl-EcoRV restriction fragment (97.5 to 98.9 mu) encodes the upstream regulatory sequences, open reading frame, and downstream sequences of the immediate early IE-N gene. Using a convient restriction site, the 285-bp promoter of IE-N was divided into two functional regions as defined by transient expression assays of mutant sequences. The sequences of IE-N from −1 to −45 nt encoded a minimal promoter capable of directing low levels of transcription. The minimal promoter was fully responsive to positive regulation by IE-N. The upstream region from −46 to −285 nt contains two direct repeats which increased levels of IE-N gene expression. Computer-assisted translation of the IE-N sequence indicates that this fragment of DNA encodes a single long open reading frame with a predicted molecular weight of 47,000. The amino acid sequence of the predicted protein exhibits three motifs common to transcriptional regulators: a serinethreonine rich region, a proline-rich region, and a polyglutamine tract. IE-N autoregulates its own expression and stimulates both IE-1 and IE-0 in transient assays. The stimulation of IE-1 may account for the augmenting activity of IE-N in the IE-1-mediated trans-activation of the 39K promoter.</div>
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Molecular analysis of a baculovirus regulatory gene

Molecular analysis of a baculovirus regulatory gene

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri